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Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Published on: June 6, 2017

Nucleoporin levels regulate cell cycle progression and phase-specific gene expression.

Papia Chakraborty1, Yaming Wang, Jen-Hsuan Wei

  • 1Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

Developmental Cell
|November 13, 2008
PubMed
Summary
This summary is machine-generated.

The Nup107-160 complex, crucial for nuclear pore function, shows cell cycle-regulated levels. Nup96 downregulation during mitosis impacts cell cycle progression and gene expression.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The Nup107-160 complex is a major component of the nuclear pore complex.
  • It plays essential roles in mRNA export, kinetochore function, spindle assembly, and nuclear pore reassembly.
  • Its constituents are known to be regulated throughout the cell cycle.

Purpose of the Study:

  • To investigate the cell cycle regulation of Nup107-160 complex constituents.
  • To determine the specific role of Nup96 in cell cycle progression.
  • To elucidate the impact of Nup96 levels on gene expression and nuclear export.

Main Methods:

  • Analysis of Nup107-160 complex constituent levels during the cell cycle.
  • Investigating Nup96 downregulation via the ubiquitin-proteasome pathway.
  • Studying cell cycle progression in cells with experimentally altered Nup96 levels (e.g., Nup96(+/-) mice).

Main Results:

  • Nup107-160 complex constituents are coordinately regulated with the cell cycle.
  • Nup96 is preferentially downregulated at mitosis through the ubiquitin-proteasome pathway.
  • Elevated Nup96 levels delay G1/S progression, while reduced levels accelerate it, causing defects in mRNA export and protein expression of cell cycle regulators.

Conclusions:

  • Nup96 levels are critical for regulating differential gene expression in a phase-specific manner.
  • Nup96 acts as a key regulator of cell cycle progression by influencing nuclear export and protein expression.
  • Proper cell cycle progression is dependent on the precise regulation of Nup96 levels during mitosis.