Related Experiment Video

Updated: Jun 28, 2026

Optimized Protocol for Generating Functional Pancreatic Insulin-secreting Cells from Human Pluripotent Stem Cells
06:33

Optimized Protocol for Generating Functional Pancreatic Insulin-secreting Cells from Human Pluripotent Stem Cells

Published on: February 2, 2024

Fresh-frozen plasma as a source of exogenous insulin-like growth factor-I in the extremely preterm infant

Ingrid Hansen-Pupp1, Eva Engström, Aimon Niklasson

  • 1Division of Pediatrics, Department of Clinical Sciences Lund, Lund University Hospital, 221 85 Lund, Sweden. ingrid.pupp@skane.se

The Journal of Clinical Endocrinology and Metabolism
|November 13, 2008
PubMed

Insights

Fresh-frozen plasma (FFP) transfusions significantly increase insulin-like growth factor-I (IGF-I) and IGF binding protein-3 (IGFBP-3) levels in extremely preterm infants. This intervention helps restore crucial proteins important for neurodevelopment and blood vessel growth.

Area of Science:

  • Neonatology
  • Pediatric Endocrinology
  • Transfusion Medicine

Background:

  • Preterm birth is associated with reduced levels of IGF-I and IGFBP-3, critical for neurogenesis and angiogenesis.
  • Maintaining adequate IGF-I and IGFBP-3 levels is vital for the development of extremely preterm infants.

Purpose of the Study:

  • To investigate the impact of fresh-frozen plasma (FFP) transfusions on circulatory IGF-I and IGFBP-3 levels in extremely preterm infants.
  • To assess the efficacy of FFP administration in normalizing key growth factor levels.

Main Methods:

  • A prospective cohort study involving 20 extremely preterm infants requiring FFP transfusion.
  • Blood samples were collected before and at multiple time points after FFP transfusion to measure IGF-I and IGFBP-3 concentrations.

Main Results:

  • FFP transfusion led to a significant increase in IGF-I (133%) and IGFBP-3 (61%) levels immediately post-transfusion.
  • Elevated levels of both IGF-I and IGFBP-3 were sustained for up to 12 hours after transfusion.
  • The estimated half-life of administered IGF-I was approximately 3.4 hours per kilogram of infant weight.

Conclusions:

  • FFP transfusion is an effective method for increasing IGF-I and IGFBP-3 levels in extremely preterm infants.
  • This intervention may support the neurodevelopmental and angiogenic processes in this vulnerable population.
Abstract

More Related Videos

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion08:34

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion

Published on: February 24, 2021

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells12:06

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Published on: January 11, 2019

Related Concept Videos

Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...