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Updated: Jun 28, 2026

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

A practical approach for intracellular protein delivery.

Claire O Weill¹, Stéphanie Biri, Abdennaji Adib

¹Polyplus-transfection, R&D Department, Bd Sebastien Brant, Bioparc, P.O. Box 90018, Illkirch Cedex, 67401, France, cweill@polyplus-transfection.com.

|November 13, 2008

Summary

This study introduces a novel non-peptide reagent for efficient protein delivery into live cells. The reagent ensures effective release of proteins into the cytoplasm, overcoming limitations of existing methods for cell biology research.

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Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

Published on: September 2, 2015

Area of Science:

Cell biology
Molecular biology
Biochemistry

Background:

Protein delivery is crucial for live-cell experiments, including protein interaction studies and functional analysis.
Current protein delivery reagents often struggle with efficient cytoplasmic release after crossing the plasma membrane.

Purpose of the Study:

To demonstrate efficient intracellular protein delivery using a novel non-peptide based reagent.
To validate effective cytoplasmic release of delivered proteins.
To provide an optimized protocol for intracellular protein delivery.

Main Methods:

Utilized a non-peptide based reagent for protein delivery in HeLa cells and primary human skin fibroblasts.
Employed fluorescent proteins for visualization and quantification.
Applied fluorescence microscopy and fluorescence-assisted cell sorting (FACS) for analysis.

Main Results:

Demonstrated efficient protein delivery and subsequent release into the cytoplasm of target cells.
Confirmed the reagent's effectiveness in both cancer cell lines (HeLa) and primary cells (fibroblasts).
Showcased successful intracellular protein delivery using fluorescence microscopy and FACS.

Conclusions:

The developed non-peptide reagent offers a superior method for intracellular protein delivery compared to existing reagents.
Effective cytoplasmic release is achievable, enhancing the utility of protein delivery for various cell biology applications.
An optimized protocol facilitates successful and reproducible intracellular protein delivery experiments.