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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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Updated: Jun 28, 2026

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
07:06

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Published on: May 14, 2020

Separation of CHO cells using hydrocyclones.

Rodrigo C V Pinto1, Ricardo A Medronho, Leda R Castilho

  • 1Federal University of Rio de Janeiro (UFRJ), COPPE - Chemical Engineering Program, Caixa Postal 68502, CEP 21941-972, Rio de Janeiro, RJ, Brazil.

Cytotechnology
|November 13, 2008
PubMed
Summary

Hydrocyclones effectively separate Chinese hamster ovary (CHO) cells with high efficiency and low viability loss. These robust devices show no signs of apoptosis induction, making them ideal for cell retention in perfusion cultures.

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Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Cell Separation Technology

Background:

  • Hydrocyclones are robust, simple separation devices with no moving parts.
  • Recent interest in applying hydrocyclones for animal cell separation.
  • Chinese Hamster Ovary (CHO) cells are crucial for biopharmaceutical production.

Purpose of the Study:

  • Investigate hydrocyclone configurations for efficient Chinese hamster ovary (CHO) cell separation.
  • Evaluate the impact of hydrocyclones on cell viability and apoptosis.
  • Develop mathematical models for predicting hydrocyclone performance.

Main Methods:

  • Experimental design to test various hydrocyclone configurations.
  • Cell separation efficiency measured using wild-type and recombinant CHO cell lines.
  • Cell viability assessed via trypan blue exclusion and lactate dehydrogenase (LDH) release.
  • Apoptosis induction monitored post-hydrocycloning.

Main Results:

  • Achieved cell separation efficiencies exceeding 97% for both CHO.K1 and recombinant CHO cell lines.
  • Observed minimal cell viability loss.
  • No significant increase in apoptotic cell concentration detected within 48 hours.
  • Developed predictive mathematical models for hydrocyclone performance.

Conclusions:

  • Hydrocyclones demonstrate high efficacy and robustness for CHO cell separation.
  • The technology is suitable for cell retention in long-term perfusion bioprocessing.
  • Absence of apoptosis induction supports the safety and applicability of hydrocyclones.