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Improved sensitivity of trypan blue dye exclusion assay with Ni2+ or Co2+ salts
1Department of Organic Chemistry and Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560 012, India.
Abstract:
A modified trypan blue dye exclusion assay was developed usingNi(2+) or Co(2+) salts to determine the viability ofprimary and transformed cells. When the cells were preincubatedwith NiSO(4) or CoCl(2) followed by trypan blue assay, thecontrast between stained and unstained cells was significantlyincreased as compared to the conventional trypan blue dyeexclusion assay.
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