Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design and synthesis of novel quinazolinone-1,2,3-triazole hybrids as new anti-diabetic agents: In vitro α-glucosidase inhibition, kinetic, and docking study.

Bioorganic chemistry·2018
Same author

Setting research priorities to achieve long-term health targets in Iran.

Journal of global health·2018
Same author

Efficacy and Safety of the Biosimilar Recombinant Human Parathyroid Hormone Cinnopar<sup>®</sup> in Postmenopausal Osteoporotic Women: A Randomized Double-blind Clinical Trial.

Iranian journal of public health·2018
Same author

The Association of Dietary Quality Indices and Cancer Mortality: A Systematic Review and Meta-analysis of Cohort Studies.

Nutrition and cancer·2018
Same author

The association between fat mass and the risk of breast cancer: A systematic review and meta-analysis.

Clinical nutrition (Edinburgh, Scotland)·2018
Same author

Novel cinnamic acid-tryptamine hybrids as potent butyrylcholinesterase inhibitors: Synthesis, biological evaluation, and docking study.

Archiv der Pharmazie·2018

Related Experiment Video

Updated: Jun 28, 2026

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
07:44

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation

Published on: April 3, 2026

Optimizing conditions for rat pancreatic islets isolation.

Mahsa M Amoli1, Roohollah Moosavizadeh, Bagher Larijani

  • 1Endocrinology & Metabolism Research Centre, Tehran University of Medical Sciences, Shariati Hospital, North Karegar St., 14114, Tehran, Iran, mahsa_amoli@hotmail.com.

Cytotechnology
|November 13, 2008
PubMed
Summary
This summary is machine-generated.

Optimizing rat islet isolation involves controlling collagenase concentration, pH, temperature, and calcium. Adding glycerol significantly reduces gelling, improving islet yield for transplantation.

More Related Videos

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
06:56

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Published on: August 30, 2019

A High-content In Vitro Pancreatic Islet &#946;-cell Replication Discovery Platform
09:35

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

Published on: July 16, 2016

Related Experiment Videos

Last Updated: Jun 28, 2026

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
07:44

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation

Published on: April 3, 2026

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
06:56

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Published on: August 30, 2019

A High-content In Vitro Pancreatic Islet &#946;-cell Replication Discovery Platform
09:35

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

Published on: July 16, 2016

Area of Science:

  • * Endocrinology and Diabetes Research
  • * Surgical and Transplantation Science

Background:

  • * Rat pancreatic islet isolation is crucial for diabetes research and transplantation studies.
  • * A major challenge is the formation of a viscous, gel-like substance during collagenase digestion, which reduces islet yield.

Purpose of the Study:

  • * To investigate factors influencing rat islet isolation and mitigate gel formation.
  • * To enhance islet yield through procedural optimization.

Main Methods:

  • * Islet isolation performed on 26 adult male Wistar Albino rats (280-350 g).
  • * Systematic examination of collagenase concentration, pH, incubation temperature, calcium ion concentration, and glycerol addition.

Main Results:

  • * Optimized collagenase concentration, pH (7.7-7.9), temperature (38±1°C), and calcium ions reduced gel formation.
  • * Glycerol addition effectively reduced or eliminated gel-like material.
  • * These modifications significantly increased rat islet yield.

Conclusions:

  • * Precise optimization of digestion parameters and the use of glycerol are key to improving rat islet isolation.
  • * Enhanced islet yield through this optimized procedure benefits islet transplantation studies.