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Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
Published on: February 16, 2015
Regulating apoptosis in mammalian cell cultures.
1Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, 21218, USA.
Cytotechnology
|November 13, 2008
Summary
Strategies to delay apoptosis, or programmed cell death, in mammalian cell cultures can significantly improve the production of therapeutic proteins. This research reviews methods to enhance cell viability and productivity in bioreactors.
Area of Science:
- Biotechnology and bioprocessing
- Mammalian cell culture for biologics production
Background:
- Cell culture technology is crucial for producing therapeutic and diagnostic proteins.
- Mammalian cells in bioreactors are integral to biological product development.
- Apoptosis (programmed cell death) limits mammalian cell culture efficiency and output.
Purpose of the Study:
- To review strategies for delaying apoptosis in mammalian cell cultures.
- To discuss methods for improving cellular viability and productivity in batch cultures.
Main Methods:
- Review of genetic strategies to block apoptotic signals.
- Review of chemical strategies to block apoptotic signals.
- Analysis of implemented methods to enhance cell viability and productivity.
Main Results:
- Both genetic and chemical approaches can effectively delay apoptosis.
- Improved cellular viability leads to increased protein product output.
- Strategies discussed aim to make biopharmaceutical production more economical.
Conclusions:
- Delaying apoptosis is key to enhancing mammalian cell culture productivity.
- Implementing strategies to improve cell longevity offers economic benefits.
- Further research into apoptosis inhibition can optimize biopharmaceutical manufacturing.
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