Over-expression of protein kinase Balpha enhances recombinant protein expression in transient systems

As more genes are being identified through genomic techniques,the need to rapidly express recombinant proteins for functionalstudies has become increasingly acute. Transient expression ofrecombinant protein using COS-1, CV-1 and 293 cells is widelyused to address this need. To improve the robustness of hostcells for transient expression, the effect of over-expression ofProtein Kinase Balpha has been explored. In this report wedemonstrate that over-expression of Protein Kinase Balpha canimprove transient recombinant protein expression 40% to >200%depending on the protein being expressed and the cell line used.