Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does radiographic imaging for spinal pain in patients receiving chiropractic care change patient outcomes? A pilot randomised controlled trial.

Chiropractic & manual therapies·2026
Same author

Healthcare Staff Resilience to Work-Related Challenges at Day Centres for Older Adults: A Longitudinal Study of Staff's Experiences Under Pressure.

Scandinavian journal of caring sciences·2026
Same author

Reconsidering Laparoscopic Peritoneal Lavage for Hinchey III Perforated Diverticulitis: A Staged Pathway Hypothesis for Selected High-Capability Centres.

ANZ journal of surgery·2026
Same author

Correction: Mindbench.ai: an actionable platform to evaluate the profile and performance of large language models in a mental healthcare context.

NPP - digital psychiatry and neuroscience·2026
Same author

Rehabilitation interventions at senior day centres for older adults: a scoping review.

Frontiers in public health·2025
Same author

Mindbench.ai: an actionable platform to evaluate the profile and performance of large language models in a mental healthcare context.

NPP - digital psychiatry and neuroscience·2025

Related Experiment Video

Updated: Jun 28, 2026

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins
08:46

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

Published on: September 22, 2020

Process development for functional membrane receptor production in mammalian cells.

Christel Fenge1, Irma Jansson, Thomas Fröberg

  • 1AstraZeneca Biotech Laboratory, 151 85, Södertälje, Sweden.

Cytotechnology
|November 13, 2008
PubMed
Summary

Optimizing cell culture conditions for G-protein coupled receptors (GPCRs) is crucial. Maximum serotonin receptor expression was achieved in late-exponential growth, enhanced by sodium butyrate and 31°C incubation, but functional activity was reduced.

More Related Videos

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
07:08

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Published on: December 23, 2015

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
07:09

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

Related Experiment Videos

Last Updated: Jun 28, 2026

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins
08:46

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

Published on: September 22, 2020

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
07:08

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Published on: December 23, 2015

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
07:09

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

Area of Science:

  • Biochemistry and Molecular Biology
  • Cell Culture and Bioprocessing
  • Receptor Pharmacology

Background:

  • G-protein coupled receptors (GPCRs) are critical drug targets.
  • CHO cells are widely used for recombinant protein expression.
  • Optimizing cell culture conditions is essential for maximizing receptor yield and function.

Purpose of the Study:

  • To characterize culture conditions for maximum expression and functional activity of serotonin (5HT) and metabotropic glutamate (mGlu) receptors in CHO cells.
  • To identify optimal harvesting times and media additives for enhanced receptor studies.

Main Methods:

  • Stable expression of 5HT and mGlu receptors in Chinese Hamster Ovary (CHO) cells.
  • Ligand binding assays to measure receptor density (5HT receptor).
  • Agonist-stimulated GTPγS binding assays to determine functional activity.
  • Varied cell culture growth phases, sodium butyrate treatment, temperature, and agitation systems.

Main Results:

  • Maximum 5HT receptor density was observed in late-exponential growth phase cells.
  • Sodium butyrate and 31°C incubation for 24 hours increased 5HT receptor expression but reduced functional activity.
  • mGlu receptor functional activity was significantly reduced in agitated culture systems but restored with medium exchange and 31°C incubation for 48 hours.

Conclusions:

  • Cell growth phase and specific culture additives (sodium butyrate, temperature) differentially impact GPCR expression and function.
  • Optimized conditions, including temperature shifts and medium exchange, can restore and enhance GPCR functional activity in membrane preparations.
  • These findings provide critical insights for maximizing the utility of GPCRs in membrane-based assays and drug discovery.