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Related Concept Videos

Plant Tissue Culture02:57

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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The only elements required by plants that are deficient in seawater are nitrogen, phosphorous and iron.

Cytotechnology·2011
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Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
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Tissue culture: the unrealized potential.

Gordon Sato1

  • 1A and G Pharmaceutical Corp., 27 Cedar St., Wenham, MA, 01984, USA, manzanarmangrove@hotmail.com.

Cytotechnology
|November 13, 2008
PubMed
Summary

Tissue culture cells do not dedifferentiate; instead, fibroblasts selectively overgrow. New techniques enable the isolation of functionally differentiated cell strains, advancing cell biology and cancer therapy research.

Area of Science:

  • Cell Biology
  • Tissue Engineering
  • Cancer Research

Background:

  • The prevailing belief in cell dedifferentiation in tissue culture hindered research.
  • Selective fibroblast overgrowth was misinterpreted as widespread dedifferentiation.

Purpose of the Study:

  • To challenge the dogma of cell dedifferentiation in vitro.
  • To develop methods for isolating functionally differentiated cell lines from mixed cultures.
  • To explore the potential of tissue culture for studying cellular behavior and discovering therapeutic targets.

Main Methods:

  • Utilized enrichment culture techniques, including alternating animal and culture passages.
  • Applied these methods to selectively favor the growth of differentiated cells over fibroblasts.
  • Developed hormonally defined media for culturing specific cell lines.

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A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies
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Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair
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Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Published on: February 24, 2016

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A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies
08:51

A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies

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Main Results:

  • Successfully isolated numerous functionally differentiated clonal cell strains.
  • Demonstrated that selective fibroblast overgrowth, not dedifferentiation, explains the loss of function in original tissue cultures.
  • Identified hormonal dependencies in cancer cells cultured in vitro.

Conclusions:

  • The hypothesis of cell dedifferentiation in tissue culture is refuted.
  • Cells in culture can accurately represent in vivo cellular states under appropriate conditions.
  • Hormonally defined media and differentiated cell lines significantly enhance tissue culture applications, offering new therapeutic strategies for cancer.