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Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System

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Published on: October 10, 2014

Insect cells respiratory activity in bioreactor.

Marilena Martins Pamboukian¹, Soraia Athie Calil Jorge, Mariza Gerdulo Santos

¹Escola Politécnica da Usp - Departamento de Engenharia Química , Av. Prof. Luciano Gualberto, trav.3 n.380 - PQI/EPUSP , São Paulo, SP, 05508-970, Brazil, mari@macsystemeduc.com.br.

|November 13, 2008

Summary

This summary is machine-generated.

This study measured the specific respiration rate of insect cell lines, finding that smaller Drosophila melanogaster (S2) cells achieve higher concentrations and lower respiration than Spodoptera frugiperda (Sf9) cells.

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Area of Science:

Biotechnology
Cell Biology
Metabolic Engineering

Background:

Specific respiration rate is crucial for understanding cell metabolism and optimizing bioprocesses.
Insect cell lines like Spodoptera frugiperda (Sf9) and Drosophila melanogaster (S2) are vital for protein and bioinsecticide production.

Purpose of the Study:

To investigate and compare the respiratory activity and oxygen transfer rates of different insect cell lines.
To analyze the impact of cell size and genetic modification on cellular respiration during growth.

Main Methods:

Cultivation of Spodoptera frugiperda (Sf9), wild Drosophila melanogaster (S2), and transfected S2 cells (S2AcGPV, S2MtEGFP) in a controlled 1-L bioreactor.
Measurement of specific respiration rate ([Formula: see text]) and cell concentration using SF900II serum-free medium.

Main Results:

Spodoptera frugiperda (Sf9) cells reached 10.7 x 10^6 cells/mL with a specific respiration rate of 7.3 x 10^-17 molO(2)/cell·s.
Drosophila melanogaster (S2) cells achieved higher concentrations (51.2 x 10^6 cells/mL) but lower respiration rates (3.1 x 10^-18 molO(2)/cell·s).
Transfected S2 cells showed intermediate cell densities and respiration rates compared to wild-type S2 and Sf9 cells.

Conclusions:

Drosophila melanogaster (S2) cells exhibit higher maximum cell densities and lower specific respiration rates than Sf9 cells, likely due to their smaller size.
Findings provide valuable data for the scale-up and process control of insect cell cultures in biopharmaceutical manufacturing.