Related Experiment Video
Updated: Jun 28, 2026

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
Published on: March 1, 2016
Adhesion, growth and detachment of cells on modified polystyrene surface
V Hendrick1, E Muniz, G Geuskens
1Animal Cell Biotechnology (, Université Libre de Bruxelles, C.P. 160/17, 1050, Brussels, Belgium.
Abstract:
By adsorbing poly(N-isopropylacrylamide) (PNIPAAm) from an aqueous solution onto oxidised polystyrene without the need for grafting the polymer to the surface, we showed here that cells(CHO-K1) adhere and grow well at 37 degrees C and are detached by lowering the temperature to 10 degrees C without any other deleterious treatment. Both bacterial culture grade polystyrene Petri dishes and polystyrene beads (120 to 250mum diameters) commercially available used in static conditions of growth were tested with similar results. The contact angle of modified Petri dishes with a water droplet increases from 36 to 58 degrees when the temperature is raised from 25 to 37 degrees C indicating change in hydrophilicity of the surface as a function of temperature.
More Related Videos
Related Concept Videos
Types of Membrane Protrusions
The microvilli, an example of stable protrusions, are finger-like projections with a...
Actin Polymerization and Cell Motility
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.
Adherens Junctions
Adherens Junctions are Dynamic
The endothelial cells...
Cell Adhesion in Plants
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose, and...
Adhesion
Capillary action is a result of water’s adhesive tendencies. When a narrow glass...

