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Updated: Jun 28, 2026
Establishment of Coloproctitis Cancer Model in Mice and Evaluation of Therapeutic Effect of Chinese Medicine
Published on: October 13, 2023
Citrus compounds inhibit inflammation- and obesity-related colon carcinogenesis in mice
Takuji Tanaka1, Yumiko Yasui, Rikako Ishigamori-Suzuki
1Kanazawa Medical University, Daigaku, Uchinada, Ishikawa, Japan. takutt@kanazawa-med.ac.jp
Abstract:
Dietary polyphenols are important potential chemopreventive natural agents. Other agents, such as citrus compounds, are also candidates for cancer chemopreventives. They act on multiple key elements in signal transduction pathways related to cellular proliferation, differentiation, apoptosis, inflammation, and obesity. This short review article provides our findings of preclinical studies on potential chemopreventive activities of dietary citrus compounds, auraptene, collinin, and citrus unshiu segment membrane (CUSM), using clitis- and obesity-related colon tumorigenesis models. Dietary feeding with auraptene and collinin at dose levels of 0.01% and 0.05% significantly lowered the incidence (50-60% reduction) and multiplicity (67-80% reduction) of colonic adenocarcinomas induced by azoxymetahene [AOM, single intraperitoneal injection of 10 mg/kg body weight (bw)] and dextran sodium sulfate (1% in drinking water). Anti-inflammatory potency of aurapene and collinin may contribute to the effects. Administration with CUSM at 3 doses in diet significantly inhibited development of aberrant crypts foci induced by 5 weekly subcutaneous injections of AOM (15 mg/kg bw) in male db/db mice: 53% inhibition by 0.02% CUSM, 54% inhibition by 0.1% CUSM, and 59% inhibition by 0.5% CUSM. CUSM treatment also decreased serum level of triglycerides. Our findings suggest that certain citrus materials are capable of inhibiting clitis- and obesity-related colon carcinogenesis.
Insights
Citrus compounds like auraptene, collinin, and CUSM show promise in preventing colon cancer. These natural agents significantly reduced tumor incidence and multiplicity in preclinical models, suggesting a role in chemoprevention.
Area of Science:
- Oncology
- Nutritional Science
- Natural Products Chemistry
Background:
- Dietary polyphenols and citrus compounds are recognized for their potential chemopreventive properties.
- These compounds influence key cellular pathways involved in cancer development, including proliferation, apoptosis, and inflammation.
- Obesity and inflammation are increasingly linked to colon carcinogenesis, highlighting the need for novel preventive strategies.
Purpose of the Study:
- To investigate the chemopreventive effects of dietary citrus compounds—auraptene, collinin, and citrus unshiu segment membrane (CUSM)—against colitis- and obesity-related colon tumorigenesis.
- To evaluate the impact of these compounds on tumor incidence, multiplicity, and specific biomarkers in preclinical models.
Main Methods:
- Preclinical studies utilizing azoxymethane (AOM) and dextran sodium sulfate (DSS)-induced colitis-related colon tumorigenesis models.
- Administration of auraptene and collinin via dietary feeding at specific concentrations.
- Evaluation of CUSM effects on aberrant crypts foci (ACF) development in AOM-induced obese mice (db/db model).
- Assessment of serum triglyceride levels following CUSM administration.
Main Results:
- Dietary auraptene and collinin significantly reduced colon adenocarcinoma incidence by 50-60% and multiplicity by 67-80% in AOM/DSS models.
- CUSM administration inhibited ACF development by 53-59% in AOM-treated obese mice, depending on the dose.
- CUSM treatment led to a decrease in serum triglyceride levels.
Conclusions:
- Certain dietary citrus compounds, including auraptene, collinin, and CUSM, demonstrate significant chemopreventive potential against colon carcinogenesis.
- The anti-inflammatory properties of auraptene and collinin may contribute to their observed effects.
- Citrus materials represent a promising source of natural agents for preventing colitis- and obesity-related colon cancers.
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