Enhancement of analyte detectability in isotachophoresis exploiting spectrophotometric diode-array detection with chemometric data processing
Related Experiment Video
Updated: Jun 28, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
Stanislav Strasík1, Mária Lucaníková, Dusan Kaniansky
1Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia. strasiks@yahoo.co.uk
Abstract:
The potentialities of capillary ITP combined with diode-array detection (DAD) with subsequent chemometric data processing have been investigated in this work. A series of different migration configurations were created using model analytes, interferents and appropriate spacers. Special attention has been paid not only to constituents migrating in fully developed ITP zones but also to the spike mode of ITP migration. The purity assessment and identity confirmation of model analytes migrating in both modes were performed by means of multivariate curve resolution and target transformation factor analysis (TTFA). Their successful applications have revealed a smart way to increase in the analytical information obtained by ITP separation even in the instance of trace analysis.
Related Concept Videos
High-Performance Liquid Chromatography: Types of Detectors
UV–Vis Spectrometers
Atomic Absorption Spectroscopy: Interference
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
Gas Chromatography: Types of Detectors-II
Tandem Mass Spectrometry
