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[Thiomorpholine transformation by the fungus Bjerkandera adusta]
Mikrobiologiia
|November 14, 2008
Summary
Researchers screened lignin-degrading fungi for thiomorpholine tolerance. Bjerkandera adusta VKM F-3477 showed the highest growth and transformed thiomorpholine into thiomorpholine sulfoxide, not utilizing it as a carbon source.
Area of Science:
- Mycology
- Environmental Microbiology
- Biochemistry
Context:
- Lignin-degrading basidial fungi are known for their enzymatic capabilities.
- Thiomorpholine derivatives (1,4-perhydrothiazines) present a unique challenge for microbial degradation.
- Understanding microbial transformation of sulfur-containing heterocycles is crucial for environmental remediation.
Purpose:
- To screen lignin-degrading basidial fungi for tolerance and growth in the presence of thiomorpholine derivatives.
- To investigate the degradation pathway and metabolic fate of thiomorpholine by a selected fungal strain.
- To develop analytical methods for quantifying thiomorpholine and its degradation products.
Summary:
- A screening identified Bjerkandera adusta VKM F-3477 as the most tolerant and fast-growing fungus in the presence of thiomorpholine derivatives.
- Quantitative analysis methods using thin-layer chromatography and high-performance liquid chromatography were established for thiomorpholine and its metabolites.
- Bjerkandera adusta VKM F-3477 transformed thiomorpholine into thiomorpholine sulfoxide, which accumulated in the medium, indicating it was not used as a carbon source. Manganese peroxidase was not directly involved in this oxidation process.
Impact:
- This study identifies a specific fungal strain capable of transforming thiomorpholine derivatives, relevant for bioremediation strategies.
- The developed analytical methods provide tools for monitoring thiomorpholine degradation in environmental or industrial settings.
- The findings contribute to the understanding of microbial metabolism of sulfur-containing heterocyclic compounds.
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