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Updated: Jun 21, 2026

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
Published on: August 31, 2014
HIV-1 and HIV-2 produce different amounts of 2-long terminal repeat circular DNA in vitro
Marie Gueudin1, Joséphine Braun, Jean-Christophe Plantier
1Laboratoire de virologie UPRES EA 2656 - Laboratoire associé du centre de référence pour VIH, CHU Charles Nicolle 76031 ROUEN Cedex, France. Marie.Gueudin@chu-rouen.fr
Abstract:
We assessed HIV-1 and HIV-2 2-long terminal repeat (LTR) circular DNA production in peripheral blood mononuclear cells, MT4-CXCR4 cells and HeLa-CXCR4-CCR5 cells in vitro, relative to the respective total amounts of HIV DNA. Whatever the cell type, HIV-2 produced a smaller total amount of DNA than HIV-1 between 6 and 96 h; HIV-2 2-LTR DNA appeared later than HIV-1 2-LTR DNA, but rapidly became more abundant. This accumulation of HIV-2 2-LTR DNA points to less efficient host cell integration relative to HIV-1.
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