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Updated: Jun 28, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
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Published on: October 27, 2017

Transcriptome analysis of the zebrafish mind bomb mutant.

Junmo Hwang1, Hyung-Soo Kim, Ji-Woong Seok

  • 1School of Life Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.

Molecular Genetics and Genomics : MGG
|November 14, 2008
PubMed
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Mind bomb (Mib) protein regulates neurogenesis by controlling Notch signaling. Zebrafish mib mutants show altered gene expression, impacting neurogenesis and potentially mesoderm development.

Area of Science:

  • Developmental biology
  • Molecular genetics
  • Cell signaling

Background:

  • Mind bomb (Mib) is crucial for Notch signaling pathway activation.
  • Mib facilitates Notch ligand endocytosis, a key regulatory step.
  • The zebrafish mib (ta52b) mutant exhibits defective ubiquitin ligase activity, leading to neurogenesis inhibition and neuronal hyperplasia.

Purpose of the Study:

  • To identify genes differentially expressed in the mib (ta52b) mutant.
  • To gain a comprehensive understanding of the transcriptome profile in mib mutants.
  • To explore the broader developmental roles of Mib beyond neurogenesis.

Main Methods:

  • Genome-wide transcriptome analysis using serial analysis of gene expression (SAGE).
  • Comparison of gene expression profiles between mib (ta52b) mutant zebrafish and wild-type siblings.

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Last Updated: Jun 28, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
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  • Bioinformatic analysis to identify significantly altered transcripts.

    • Main Results:

    • Identification of 335 transcripts with significantly altered expression in the mib (ta52b) mutant compared to wild-type.
    • The mib (ta52b) mutation affects genes involved in neurogenesis.
    • Evidence suggests the mib (ta52b) mutation also impacts mesoderm development.

    Conclusions:

    • The study provides a detailed transcriptome profile of the mib (ta52b) mutant.
    • Findings reveal novel insights into the role of Mind bomb in regulating both neurogenesis and mesoderm development.
    • These results contribute to a deeper understanding of Notch signaling pathway regulation.