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Updated: Jun 28, 2026
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
Isolation and structural elucidation of polar photometabolites
J Burhenne1, M Ludwig, M Spiteller
1Department of Ecological Chemistry and Ecotoxicology, University of Kassel, Nordbahnhofstrasse la, D-37213, Witzenhausen, Germany.
Abstract:
Subsequent to irradiation with a xenon lamp simulating sunlight, fluoroquinolone carboxylic acids in aqueous solution form polar pyridone dicarboxylic and tricarboxylic acids. After liquid/liquid partition with chloroform/water these substances can be isolated by ion exchange chromatography of the aqueous phase. They can be regarded as intermediate compounds on the route to a complete photomineralization. The structural elucidation is performed by such mass spectroscopic methods as MS, GC/MS and HPLC/MS, whereby HPLC/MS shows the highest reliability. Additionally(1)H- and(13)C-NMR measurements confirm the structure of the main polar degradation product.
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