Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

B cell activating factor (BAFF) levels are associated with failed remission after 12 months of treatment with benralizumab and mepolizumab in severe asthma.

Respiratory research·2026

Same author

S100-alarmins, antenatal corticosteroids and the risk of late-onset sepsis in preterm infants: A prospective cohort study.

PloS one·2026

Same author

Immune activation and response dynamics of human iPSC-derived macrophages in tuberculosis infection models.

Clinical & translational immunology·2026

Same author

NLRP3 regulates epithelial barrier integrity and protects from airway hyperresponsiveness in experimental allergic asthma.

Frontiers in immunology·2025

Same author

Digital Health Innovation by Design: A Logic Model Scaffold for Rural, Regional, and Remote Settings.

International journal of environmental research and public health·2025

Same author

Exploring trends and predictors of long-term asthma remission.

The World Allergy Organization journal·2025

Same journal

A Modular High-Parameter Flow Cytometry Framework: Pre-Analytical Optimization and Validation for Clinical Research.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026

Same journal

Quantitative Detection of Entotic Cell-In-Cell Structures Using Deformable Segmentation and Deep Learning.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026

Same journal

Comparison of Tissue Preparations to Identify and Phenotype T Cells in Human Colorectal Tumor Tissue.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026

Same journal

Refractive Index-Correlated Pseudocoloring for Adaptive Color Fusion in Holotomographic Cytology.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026

Same journal

Ensembling Unets for Rare Chromosomal Aberration Detection in Metaphase Images, Uncertainty Quantification, and Ionizing Radiation Dose Estimation.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026

Same journal

OMIP-121: Immune Phenotyping of Canine Peripheral Leukocytes by Mass Cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

A versatile platform for comprehensive chip-based explorative cytometry.

Christian Hennig¹, Nico Adams, Gesine Hansen

¹Department of Paediatric Pneumology and Neonatology, Hannover Medical School, Hannover, Germany. hennig.christian@mh-hannover.de

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|November 14, 2008

Summary

This summary is machine-generated.

This study introduces a novel microfluidic chip method for analyzing immune cells, significantly improving sensitivity and enabling deep immunophenotyping on small samples. This technique overcomes limitations in current immune cell analysis, offering new diagnostic and research possibilities.

More Related Videos

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Related Experiment Videos

Last Updated: Jun 28, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Area of Science:

Immunology
Microfluidics
Cellular Analysis

Background:

Current immune cell analysis methods face technical limitations, especially for multiparameter and functional analysis of small cell samples.
Comprehensive immunophenotyping is hampered by the complexity of the immune system and existing technological constraints.

Purpose of the Study:

To present a novel method for analyzing living immune cells using microfluidic chips and automated microscopy.
To overcome limitations in current methodologies for multiparameter and functional analysis of small cell samples.
To demonstrate the scientific and diagnostic potential of this chip-based cytometry.

Main Methods:

Developed a method for stepwise functional manipulation and analysis of living immune cells immobilized in microfluidic chips.
Utilized automated epifluorescence microscopy for analysis.
Validated the method against flow cytometry, assessing sensitivity and specificity.
Applied the method to analyze rare cells, limited sample volumes, and in vitro differentiated human B-cells.

Main Results:

Achieved 10-fold increased sensitivity compared to flow cytometry, with comparable specificity.
Enabled analysis of a virtually unlimited number of intracellular and surface markers on living immune cells.
Successfully identified and phenotyped rare cells and performed deep immunophenotyping on limited sample volumes.

Conclusions:

The chip-based cytometry method overcomes current limitations for comprehensive immunophenotyping, especially for precious samples with few cells.
The technique allows for detailed analysis of numerous markers on living immune cells using small sample volumes.
This method offers a valuable tool for exploring small cell-containing samples with high marker capacity, with potential applications in diagnostics and research.