[Rapid determination of thiabendazole residue in barbary wolfberry fruit by ion exchange chromatography and fluorescence detection]
Jie Zhao1, Qiang He, Xiang-hong Kong
1College of Science, Xi'an University of Technology, Xibn 710048, China. redflyingbird@yahoo.com.cn
Objective:
To developed a method for the determination of thiabendazole residues in barbary wolfberry fruit by ion exchange chromatography (IEC) with fluorescence detection.
Method:
The samples were extracted with 0.1% H3PO4. Chromatographic analysis was performed on a Supelcosil LC-SCX (4.6 mm x 250 mm, 5 microm) column eluted with 0.1 mol x L(-1) KH2PO4 (pH 3.0)-acetonitrile (70:30) and detection at lamdaex = 307 nm and lamdaem = 359 nm.
Result:
Thiabendazole in measured samples was separated completely. The calibration curve was linear at the range of 0.0005-0.02 mg x L(-1) with good precision and accuracy.
Conclusion:
The proposed method was satisfactorily applied to the analysis of thiabendazole residues in barbary wolfberry fruit.
