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Published on: July 3, 2020
[Effect of estrogen on heat shock protein 70 expression in rat masseter muscle]
Bing-yan Li1, Yu Zhao, Xiao-qing Li
1State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the effect of estrogen on heat shock protein (HSP) 70 expression in rat masseter muscle.
Methods:
Sixty twelve-week-old female Sprague-Dawley rats were randomly divided into three groups: Sham surgery group (control group), ovariectomy group (OVX group), ovariectomy with estradiol valerate replacement treatment group (OVX/EV group). Half of the animals were sacrificed at 4 and 8 weeks respectively, then the masseter muscle was removed. Immunohistochemistry (IHC) method was employed to study the HSP70 expression in masseter muscle.
Results:
Compared to control group and OVX/EV group, the expression of HSP70 was significantly lower at 8 weeks in OVX group (P < 0.05). There were no significantly difference between the HSP70 expression of control group and that of OVX/EV group.
Conclusion:
Estrogen may affect HSP70 expression in rat masseter muscle, and estrogen replacement therapy may prevent HSP70 reduction.
