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Human T-lymphocytes synthesize and secrete a protease resistant proteoglycan in a delayed, serum-dependent response
R Chopra1, E Griffith, D Irwin
1Department of Medicine, Queen's University, Kingston, Ontario, Canada.
Cell Biology International Reports
|January 1, 1991
Abstract:
In the presence of radioactive precursors, the addition of Concanavalin A (Con A) to cultures of mononuclear cells (MC) from human blood resulted in marked accumulation in the medium of a protease-resistant proteoglycan (PG) with chondroitin sulfate (CS) chains of an average molecular weight of 28 KDa. This PG was shown to be elaborated primarily by T-cells. The addition of serum was necessary for maximal stimulation with Con A and under these conditions PG accumulation in the medium rose slowly during the first few days of culture and much more rapidly between days 6-8.