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Updated: Jun 28, 2026

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

Published on: August 16, 2014

Chapter 6. Ocular models of angiogenesis.

Edith Aguilar¹, Michael I Dorrell, David Friedlander

¹Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA.

Methods in Enzymology

|November 15, 2008

Summary

This study reviews methods for studying ocular angiogenesis, the process of blood vessel formation in the eye. Understanding these processes is key to treating vision loss from diseases like retinopathy.

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Last Updated: Jun 28, 2026

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

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An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

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Area of Science:

Ophthalmology
Vascular Biology
Cell Biology

Background:

Normal ocular angiogenesis involves ordered cell proliferation and migration.
Abnormal angiogenesis in the eye leads to dysfunctional blood vessels and vision loss.
Diseases like ischemic retinopathies and choroidal neovascularization involve pathological angiogenesis.

Purpose of the Study:

To outline protocols for studying ocular angiogenesis.
To provide historical context for these experimental methods.
To describe research questions addressed by these protocols.

Main Methods:

Review of established experimental models for ocular angiogenesis.
Description of protocols for studying normal and pathological neovascularization.
Historical context of angiogenesis research in ophthalmology.

Main Results:

Multiple experimental models exist to study ocular angiogenesis.
These models recapitulate aspects of normal and pathological neovascularization.
Protocols are available to study vascular leak and choroidal neovascularization.

Conclusions:

Experimental models are crucial for understanding ocular angiogenesis.
Studying ocular angiogenesis aids in developing treatments for vision-threatening diseases.
Protocols discussed are valuable for diverse research in ophthalmology.