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Related Experiment Video

Updated: Jun 28, 2026

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy
05:08

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Published on: September 11, 2013

Chapter 14. Measuring intratumoral microvessel density.

Noel Weidner1

  • 1Department of Pathology, University of California, San Diego, San Diego, California, USA.

Methods in Enzymology
|November 15, 2008
PubMed
Summary
This summary is machine-generated.

Tumor growth requires new blood vessel formation (angiogenesis). Measuring intratumoral microvessel density (iMVD) helps assess tumor aggressiveness and potential therapeutic targets.

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Related Experiment Videos

Last Updated: Jun 28, 2026

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy
05:08

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Published on: September 11, 2013

Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research
07:26

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Published on: September 15, 2023

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Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

Published on: January 19, 2018

Area of Science:

  • Oncology
  • Vascular Biology
  • Cancer Research

Background:

  • Tumor growth beyond 1-2 mm³ necessitates angiogenesis, the formation of new blood vessels.
  • Tumor cells and host vasculature form an integrated ecosystem, with tumor-derived signals promoting endothelial cell proliferation.
  • Anti-angiogenesis strategies are recognized as a potential anti-cancer therapy.

Purpose of the Study:

  • To review techniques for measuring intratumoral microvessel density (iMVD).
  • To highlight the correlation between iMVD and aggressive tumor behavior.

Main Methods:

  • Review of established and emerging techniques for quantifying microvessel density within tumors.
  • Focus on identifying and analyzing vascular "hot spots" for accurate iMVD assessment.

Main Results:

  • Evidence confirms tumor growth is angiogenesis-dependent.
  • Tumor cells produce angiogenic regulatory molecules.
  • Angiogenesis inhibitors have demonstrated efficacy in slowing tumor growth.
  • Intratumoral microvessel density (iMVD) in vascular hot spots correlates with tumor aggressiveness.

Conclusions:

  • Angiogenesis is a critical and validated target for anti-cancer therapies.
  • Accurate measurement of iMVD is crucial for predicting tumor behavior and guiding treatment strategies.