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Updated: Jun 28, 2026

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
Published on: January 25, 2020
Eukaryotic gene regulation in three dimensions and its impact on genome evolution
M Madan Babu1, Sarath Chandra Janga, Ines de Santiago
1MRC Laboratory of Molecular Biology, Cambridge, UK. madanm@mrc-lmb.cam.ac.uk
Abstract:
Recent advances in molecular techniques and high-resolution imaging are beginning to provide exciting insights into the higher order chromatin organization within the cell nucleus and its influence on eukaryotic gene regulation. This improved understanding of gene regulation also raises fundamental questions about how spatial features might have constrained the organization of genes on eukaryotic chromosomes and how mutations that affect these processes might contribute to disease conditions. In this review, we discuss recent studies that highlight the role of spatial components in gene regulation and their impact on genome evolution. We then address implications for human diseases and outline new directions for future research.
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