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Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
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Clinical Applications of Epidermal Stem Cells

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Related Experiment Video

Updated: Jun 28, 2026

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
10:43

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Published on: December 23, 2022

Role for EPS8 in squamous carcinogenesis.

Huixin Wang1, Vyomesh Patel, Hiroshi Miyazaki

  • 1Philips Institute of Oral and Craniofacial Molecular Biology, Virginia Commonwealth University, Richmond, VA 23298-0566, USA.

Carcinogenesis
|November 15, 2008
PubMed
Summary
This summary is machine-generated.

The signaling intermediate EPS8 (Epidermal Growth factor Protein 8) drives tumor progression and metastasis in squamous cell carcinoma. Elevated EPS8 expression correlates with increased cell motility, invasion, and tumorigenicity, highlighting its role in cancer development.

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Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
07:29

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Epidermal Growth factor Protein 8 (EPS8) is a signaling intermediate implicated in cell growth and motility.
  • Its precise role in squamous cell carcinoma (SCC) progression remains to be fully elucidated.

Purpose of the Study:

  • To investigate the function of EPS8 in SCC tumor progression using in vitro and in vivo models.
  • To determine the relationship between EPS8 expression and tumor cell behavior, including migration, invasion, and tumorigenicity.

Main Methods:

  • Utilized retroviral transduction to overexpress EPS8 in low-EPS8 expressing HN4 cells (HN4/EPS8).
  • Employed short hairpin RNA (shRNA) to knock down EPS8 expression in high-EPS8 expressing HN12 cells.
  • Assessed cell proliferation, migration, and invasion in vitro.
  • Quantified matrix metalloprotease (MMP)-9 expression and activity.
  • Evaluated tumorigenicity in vivo using orthotopic transplantation assays.
  • Correlated EPS8 and MMP-9 expression in clinical SCC samples.

Main Results:

  • HN4/EPS8 cells exhibited increased proliferation, migration, and MMP-9 expression/activity, dependent on protein kinase B (AKT) signaling.
  • EPS8 knockdown in HN12 cells reduced migration, invasion, MMP-9 expression/activity, and MMP-9 promoter activity.
  • EPS8 knockdown decreased in vivo tumorigenicity.
  • EPS8 overexpression in HN4 cells induced tumorigenicity.
  • Clinical SCC samples showed variable EPS8 expression paralleling MMP-9 levels.

Conclusions:

  • EPS8 plays a crucial role in promoting SCC progression by enhancing cell motility, invasion, and tumorigenicity.
  • EPS8 influences MMP-9 expression and activity, contributing to the invasive phenotype.
  • EPS8 is a potential therapeutic target for squamous cell carcinoma.