Simultaneous detection of anti-HCV antibody and HCV core antigen
Filippo Ansaldi1, Giancarlo Icardi
1Department of Health Sciences, University of Genoa, Genoa, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2008
Abstract:
HCV infection is usually diagnosed by means of an enzyme immune assay for the detection of antibody against HCV. The window period between infection and seroconversion remains a dramatic problem in the transfusional and diagnostic setting. In this chapter, we report (i) procedures for assays using two different approaches designed to reduce the window period and (ii) performance in terms of specificity and sensitivity in the detection of both antibody and antigen, and we compare their efficacy with that of commercial assays.
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