Cellular receptors and HCV entry

Here, we present a protocol that examines specific steps of the viral entry to identify and evaluate novel antiviral...

This video demonstrates an assay to evaluate antiviral agents that target specific steps of the viral entry into host cells and fusion with the endosome. The cells are incubated at a low temperature and then infected with HCV. The low temperature permits viral binding to host cells but inhibits internalization via endocytosis. The test antiviral compound is added, and the cells are incubated at physiological temperature. This shift to a higher temperature facilitates viral entry and allows...

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.

Overview

Global Market Entry Strategies: Exporting, Licensing, and Contract Manufacturing
Global expansion requires careful selection of entry strategies based on a firm͇s goals, market conditions, and capabilities. Low-risk strategies such as exporting, licensing, and contract manufacturing offer firms various ways to enter foreign markets while mitigating potential financial exposure.
Exporting is a common entry method and can be divided into indirect and direct exporting. In indirect exporting,...