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Related Experiment Video

Updated: Jun 28, 2026

Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula

Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula

Published on: January 7, 2019

Selection of transformed plants.

Huw D Jones¹, Caroline A Sparks

¹Department of Plant Sciences, Rothamsted Research, Centre for Crop Genetic Improvement, Harpenden, Hertfordshire, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 15, 2008

Summary

Selectable marker genes are essential for plant transformation due to rare transgene integration events. This study demonstrates glufosinate herbicide resistance genes as effective selectable markers in wheat transformation using Agrobacterium or biolistics.

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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

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Last Updated: Jun 28, 2026

Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula

Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula

Published on: January 7, 2019

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Area of Science:

Plant biotechnology
Molecular biology
Genetics

Background:

Transgene integration into plant genomes is infrequent and random.
Recovering whole transgenic plants from rare integration events is challenging.
Selectable marker genes are routinely used to identify transformed plant cells.

Purpose of the Study:

To evaluate the efficacy of glufosinate herbicide resistance genes as selectable markers in wheat transformation.
To demonstrate the utility of these markers with both Agrobacterium-mediated and biolistic transformation methods.

Main Methods:

Wheat transformation experiments were conducted using Agrobacterium and biolistics.
Genes conferring resistance to glufosinate herbicides were employed as selectable markers.
Selection of transformed cells was based on herbicide resistance.

Main Results:

Glufosinate resistance genes proved effective in selecting transformed wheat cells.
The selectable markers were successfully applied in both Agrobacterium and biolistic transformation systems.
This approach facilitates the recovery of transgenic wheat lines.

Conclusions:

Genes conferring glufosinate herbicide resistance are valuable tools for wheat transformation.
These selectable markers simplify the process of generating transgenic wheat for research.
The study validates a common practice in plant genetic engineering.