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Related Experiment Video

Updated: Jun 28, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Barley transformation using biolistic techniques.

Wendy A Harwood¹, Mark A Smedley

¹Department of Crop Genetics, John Innes Centre, Colney, Norwich, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 15, 2008

Summary

This summary is machine-generated.

Microprojectile bombardment, a biolistic technique, efficiently transforms cereal crops like barley by delivering DNA-coated gold particles into plant cells. While Agrobacterium-mediated methods are now preferred, biolistics remain crucial for specific barley applications and transient assays.

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Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula

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Published on: January 7, 2019

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Last Updated: Jun 28, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula

Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula

Published on: January 7, 2019

Area of Science:

Plant biotechnology
Molecular biology
Genetics

Background:

Microprojectile bombardment (biolistics) is a key technique for cereal transformation.
Historically, biolistics enabled the first fertile transgenic barley plants.
Agrobacterium-mediated transformation is now the predominant method for many cereals, including barley.

Purpose of the Study:

To describe methods for barley transformation using biolistic procedures.
To highlight the utility of biolistic technology in transient assays for construct validation.

Main Methods:

Utilizing microprojectile bombardment to accelerate gold particles coated with plasmid DNA into plant cells.
Employing biolistic procedures for direct DNA introduction in barley transformation.
Implementing transient assays for rapid construct evaluation.

Main Results:

Biolistic techniques were foundational for early successes in creating transgenic barley.
These methods continue to be valuable for specific barley transformation needs.
Biolistics serve as effective systems for transiently assessing DNA constructs.

Conclusions:

Biolistic transformation remains a relevant technique for certain barley applications.
The chapter provides detailed methods for barley biolistic transformation.
Biolistic technology is essential for rapid transient assays in plant science.