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Related Experiment Video

Updated: Jun 28, 2026

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Published on: March 29, 2019

Barley transformation using Agrobacterium-mediated techniques.

Wendy A Harwood¹, Joanne G Bartlett, Silvia C Alves

¹Department of Crop Genetics, John Innes Centre, Colney, Norwich, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 15, 2008

Summary

This summary is machine-generated.

Agrobacterium-mediated transformation is an efficient method for creating transgenic barley. This technique yields fertile plants, ideal for studying gene function in cereal crops.

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Area of Science:

Plant Science
Biotechnology
Genetics

Background:

Agrobacterium-mediated transformation methods for barley have existed for a decade.
Agrobacterium offers superior efficiency and plant quality compared to biolistic methods.

Purpose of the Study:

To describe a simple and effective Agrobacterium-mediated transformation system for barley.
To facilitate the production of numerous fertile transgenic barley lines for research.

Main Methods:

Infection of immature barley embryos with Agrobacterium tumefaciens.
Selection of transformed tissues using hygromycin antibiotic resistance.
Regeneration of fertile transgenic barley plants.

Main Results:

The described method enables the production of a large number of independent transgenic barley lines.
Transgenic plants produced are fertile and suitable for further studies.
This system provides a reliable tool for barley genetic research.

Conclusions:

The presented Agrobacterium-mediated transformation protocol is efficient for barley.
This method is highly suitable for functional genomics studies in cereals.
It offers a practical approach for generating transgenic barley resources.