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Related Experiment Video

Updated: Jun 28, 2026

Cost-Efficient Transcriptomic-Based Drug Screening

Cost-Efficient Transcriptomic-Based Drug Screening

Published on: February 23, 2024

Establishing substantial equivalence: transcriptomics.

María Marcela Baudo¹, Stephen J Powers, Rowan A C Mitchell

¹Centre for Crop Genetic Improvement, Department of Plant Sciences, Rothamsted Research, Harpenden, Hertfordshire, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 15, 2008

Summary

Transgenic wheat expressing gluten genes showed no unintended effects on gene expression. This demonstrates substantial equivalence to conventional wheat, aiding regulatory approval for genetically modified crops.

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Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

Area of Science:

Agricultural Biotechnology
Molecular Biology
Genomics

Background:

European regulatory bodies mandate substantial equivalence for transgenic crop approval.
Transcriptomic profiling is a key method for assessing unintended effects of genetic modification.
Wheat transformation studies are crucial for understanding gene expression in genetically modified organisms.

Purpose of the Study:

To establish protocols for transcriptomic comparisons in transgenic wheat.
To assess the substantial equivalence of transgenic wheat lines expressing gluten proteins.
To identify any unintended effects of transgene integration and expression.

Main Methods:

Detailed protocols for transcriptomic analysis of developing wheat grain and leaf tissues.
Comparative analysis of gene expression profiles between transgenic and conventionally bred wheat lines.
Utilizing endosperm-specific promoters to control transgene expression.

Main Results:

Transgenic wheat lines expressing additional gluten protein genes were analyzed.
No significant unpredicted effects on endogenous gene expression were observed.
Marker genes also did not cause significant unintended alterations in gene expression.

Conclusions:

The studied transgenic wheat lines are substantially equivalent to their conventional counterparts.
Transcriptomic profiling effectively identifies unintended effects in genetically modified crops.
The findings support the commercial approval of these transgenic wheat lines.