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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Establishing substantial equivalence: proteomics.

Alison Lovegrove1, Louise Salt, Peter R Shewry

  • 1Department of Plant Sciences, Rothamsted Research, Centre for Crop Genetic Improvement, Harpenden, Hertfordshire, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2008
PubMed
Summary

Standardized proteomic analysis using two-dimensional gel electrophoresis (2D PAGE) ensures reproducible results for genetically modified wheat, assessing its substantial equivalence to conventionally bred varieties.

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Published on: March 13, 2014

Area of Science:

  • Agricultural Science
  • Biotechnology
  • Proteomics

Background:

  • Wheat is a globally significant agricultural crop, processed into numerous food products.
  • Assessing the safety and equivalence of genetically modified (GM) wheat to conventional varieties is crucial.
  • Proteomic analysis offers a method to evaluate the consequences of wheat transgenesis.

Purpose of the Study:

  • To establish reproducible standard operating procedures for proteomic analysis of wheat grain proteins.
  • To ensure the substantial equivalence of genetically modified wheat lines compared to conventionally bred ones.
  • To address the challenges of inter-laboratory reproducibility in two-dimensional PAGE (2D PAGE).

Main Methods:

  • Development of standardized operating procedures for wheat grain protein extraction.
  • Application of two-dimensional polyacrylamide gel electrophoresis (2D PAGE) for proteomic profiling.
  • Focus on ensuring reproducibility in proteomic analysis across different laboratory settings.

Main Results:

  • Established robust methods for reproducible proteomic analysis of wheat grain.
  • Demonstrated the utility of 2D PAGE for comparative analysis of wheat proteomes.
  • Provided a framework for consistent evaluation of GM wheat.

Conclusions:

  • Reproducible proteomic analysis of wheat is achievable with standardized methods.
  • This approach supports the rigorous assessment of genetically modified wheat.
  • Standardized 2D PAGE contributes to ensuring the safety and equivalence of GM crops.