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Receptor for activated C-kinase 1, a novel binding partner of adiponectin receptor 1

Yizhou Xu1, Ningfu Wang, Feng Ling

  • 1Department of Cardiology, Hangzhou the First People's Hospital, 261 Wansha Road, Hangzhou 310006, China.

Biochemical and Biophysical Research Communications
|November 18, 2008
PubMed

Insights

Receptor for activated protein kinase C1 (RACK1) interacts with adiponectin receptor 1 (AdipoR1). This interaction is crucial for adiponectin signaling and glucose uptake, suggesting RACK1 is a key bridging factor.

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Cell Signaling

Background:

  • Adiponectin is a hormone from adipose tissue with insulin-sensitizing and anti-diabetic effects.
  • Two adiponectin receptors, AdipoR1 and AdipoR2, have been identified, but their signaling pathways are not fully understood.

Purpose of the Study:

  • To identify proteins interacting with human AdipoR1.
  • To elucidate the role of interacting proteins in adiponectin signaling.

Main Methods:

  • Yeast two-hybrid screen to identify interacting proteins.
  • Co-immunoprecipitation and co-localization studies in mammalian cells.
  • RNA interference (RNAi) to knock down RACK1 expression.

Main Results:

  • Receptor for activated protein kinase C1 (RACK1) was identified as an interacting partner of AdipoR1.
  • RACK1 interaction with AdipoR1 was confirmed and enhanced by adiponectin.
  • Knockdown of RACK1 inhibited adiponectin-stimulated glucose uptake in HepG2 cells.

Conclusions:

  • RACK1 interacts with AdipoR1.
  • RACK1 may function as a bridging factor in adiponectin signal transduction.
  • RACK1 plays a role in adiponectin-mediated glucose uptake.

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