Related Experiment Video
Updated: Jun 28, 2026

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
Published on: August 9, 2019
Human multipotent stromal cells from bone marrow and microRNA: regulation of differentiation and leukemia inhibitory
Adam Z Oskowitz1, Jun Lu, Patrice Penfornis
1Center for Gene Therapy and Department of Pharmacology, Tulane University Health Sciences Center, New Orleans, LA 70112, USA.
Abstract:
We observed that microRNAs (miRNAs) that regulate differentiation in a variety of simpler systems also regulate differentiation of human multipotent stromal cells (hMSCs) from bone marrow. Differentiation of hMSCs into osteoblasts and adipocytes was inhibited by using lentiviruses expressing shRNAs to decrease expression of Dicer and Drosha, two enzymes that process early transcripts to miRNA. Expression analysis of miRNAs during hMSC differentiation identified 19 miRNAs that were up-regulated during osteogenic differentiation and 20 during adipogenic differentiation, 11 of which were commonly up-regulated in both osteogenic and adipogenic differentiation. In silico models predicted that five of the up-regulated miRNAs targeted leukemia inhibitory factor (LIF) expression. The prediction was confirmed for two of the miRNAs, hsa-mir 199a and hsa-mir346, in that over-expression of the miRNAs decreased LIF secretion by hMSCs. The results demonstrate that differentiation of hMSCs is regulated by miRNAs and that several of these miRNAs target LIF.
Related Concept Videos
Regulation of Hematopoietic Stem Cells
Lineage Commitment
Differentiation of Common Myeloid Progenitor Cells
Multipotency of Hematopoietic Stem Cells
Role of Hematopoietic Growth Factors
Thrombopoietin (TPO), mainly released by the liver,...
Mesenchymal Stem Cells

