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Related Experiment Videos

Pteroylpolyglutamates in human milk.

D L O'Connor1, T Tamura, M F Picciano

  • 1Division of Applied Human Nutrition, University of Guelph, Ontario, Canada.

The American Journal of Clinical Nutrition
|April 1, 1991
PubMed
Summary
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Microbiological assays for human milk folate require folate conjugase to accurately measure total folate and pteroylpolyglutamate (PteGlun) content. Omitting this enzyme leads to significant underestimation of folate levels.

Area of Science:

  • Nutritional Biochemistry
  • Human Milk Composition
  • Microbiological Assays

Background:

  • Inconsistent values for total folate and pteroylpolyglutamate (PteGlun) in human milk necessitate investigation into methodological variations.
  • Accurate quantification of human milk folate is crucial for understanding infant nutrition and folate bioavailability.

Purpose of the Study:

  • To identify methodological factors contributing to the variability in reported human milk folate and PteGlun content.
  • To determine the impact of folate conjugase and heating on folate measurements in human milk.

Main Methods:

  • Microbiological determination of milk folate content in two laboratories, with and without the addition of folate conjugase.
  • Assessment of PteGlun as a fraction of total folate.

Related Experiment Videos

  • Evaluation of the effect of rennin and heat treatment on folate concentrations.

    • Main Results:

    • No significant inter-laboratory differences were observed in total folate or PteGlun content.
    • PteGlun constituted a substantial portion (28%) of total folate in human milk.
    • Heating human milk significantly increased measured folate concentrations, indicating folate release from binding proteins.

    Conclusions:

    • Microbiological assays lacking folate conjugase underestimate total human milk folate due to insufficient autolysis of endogenous PteGlun.
    • Folate binding proteins must be addressed to ensure accurate folate quantification in human milk.
    • Standardized methods incorporating folate conjugase are essential for reliable human milk folate analysis.