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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

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Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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High-throughput quantitative analysis by desorption electrospray ionization mass spectrometry.

Nicholas E Manicke1, Thomas Kistler, Demian R Ifa

  • 1Department of Chemistry, Purdue University, Bindley Biosciences Center, West Lafayette, Indiana, USA.

Journal of the American Society for Mass Spectrometry
|November 18, 2008
PubMed
Summary

A new high-throughput desorption electrospray ionization (DESI) source enables rapid quantitative analysis of pharmaceuticals in complex matrices. This method offers high accuracy and precision, with low limits of detection, making it suitable for various applications.

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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • High-throughput analysis is crucial for pharmaceutical research.
  • Desorption electrospray ionization (DESI) offers potential for rapid sample analysis.
  • Characterizing new DESI sources is essential for optimizing quantitative performance.

Purpose of the Study:

  • To characterize a novel high-throughput DESI source for quantitative analysis.
  • To evaluate the performance of the DESI source in terms of accuracy, precision, and limit of detection (LOD).
  • To assess the DESI source's applicability to diverse matrices, including biological samples.

Main Methods:

  • A 96-sample array of pharmaceuticals was analyzed using a new high-throughput DESI source.
  • Quantitative performance was assessed using propranolol (PRN) and carbamazepine (CBZ) with deuterated internal standards (IS).
  • Samples were analyzed in both chemical background-free conditions and complex matrices (urine, lipid extract).

Main Results:

  • The DESI source achieved a total analysis time of 3 minutes for a 96-sample array.
  • Background-free samples showed excellent quantitative accuracy (RE < 3%) and precision (RSD < 5%).
  • Analysis in urine and lipid extract yielded good accuracy (RE < 3%) and acceptable precision (RSD ~10% and 4%, respectively).
  • LODs for PRN and CBZ were as low as 10 and 30 fmol in background-free conditions, increasing in complex matrices.

Conclusions:

  • The developed high-throughput DESI source demonstrates robust performance for quantitative analysis of pharmaceuticals.
  • The system is effective across various matrices, including challenging biological samples.
  • This technology offers a rapid and accurate method for high-throughput quantitative measurements.