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Related Experiment Video

Updated: Jun 27, 2026

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
14:57

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Published on: March 23, 2011

Age-related decrease in constructive activation of Akt/PKB in SAMP10 hippocampus.

Kun Nie1, Jian-Chun Yu, Yu Fu

  • 1Tianjin Key Laboratory of Acupuncture and Moxibustion, Acupuncture and Moxibustion Research Institute, The First Hospital affiliated to Tianjin College of Traditional Chinese Medicine, 314 West Anshan Avenue, Tianjin 300193, PR China.

Biochemical and Biophysical Research Communications
|November 18, 2008
PubMed
Summary

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Aging accelerates neurodegenerative diseases like Alzheimer's. This study shows age-related decreases in Akt signaling correlate with memory decline in mice, suggesting a link to cognitive impairment.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Aging Research

Background:

  • Aging is the primary risk factor for neurodegenerative diseases, including Alzheimer's disease (AD).
  • Age-dependent changes in cell signaling pathways are implicated in AD pathogenesis.
  • The Akt signaling pathway is crucial for neuronal survival.

Purpose of the Study:

  • To investigate the impact of aging on Akt signaling in the hippocampus.
  • To explore the relationship between age-related Akt signaling alterations and cognitive function.

Main Methods:

  • Utilized the senescence-accelerated mouse model (SAMP10) and a control strain (SAMR1).
  • Assessed Akt mRNA and protein expression levels.
  • Measured Akt phosphorylation at Serine 473 (Akt(Ser473)) using Western blotting or similar techniques.

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Last Updated: Jun 27, 2026

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
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Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Published on: March 23, 2011

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07:44

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Published on: October 6, 2017

  • Evaluated learning and memory performance in mice using behavioral tasks.

    • Main Results:

    • Akt mRNA and protein expression remained stable during aging in SAMP10 mice.
    • A continuous decrease in Akt(Ser473) phosphorylation was observed after 6 months of age in SAMP10 mice.
    • Aged SAMP10 mice exhibited significantly reduced Akt(Ser473) phosphorylation compared to control SAMR1 mice.
    • SAMP10 mice at 6 months of age showed impaired performance in learning and memory tasks.

    Conclusions:

    • Age-related decline in Akt(Ser473) phosphorylation occurs in the hippocampus of SAMP10 mice.
    • These findings suggest a potential association between altered Akt(Ser473) signaling and age-related memory deficits in this model.