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Related Experiment Video

Updated: Jun 27, 2026

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

Liposome encapsulated polyethylenimine/ODN polyplexes for brain targeting.

Young Tag Ko¹, Raktima Bhattacharya, Ulrich Bickel

¹Department of Pharmaceutical Sciences, Texas Tech University Health Science Center, Amarillo, 79106, USA.

Journal of Controlled Release : Official Journal of the Controlled Release Society

|November 18, 2008

Summary

PEGylated liposomes encapsulate polyethylenimine/oligodeoxynucleotide polyplexes, enhancing systemic circulation and reducing RES uptake. This novel delivery system shows potential for targeted in vivo nucleic acid delivery, including to the brain.

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Assembly and Characterization of Polyelectrolyte Complex Micelles

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Area of Science:

Biotechnology
Nanomedicine
Drug Delivery Systems

Background:

Cationic polymer polyethylenimine (PEI) shows high in vitro transfection but limited systemic application due to rapid blood clearance and RES accumulation.
Improving PEI/DNA polyplex properties is crucial for extended circulation time and suppressed RES uptake in systemic delivery.

Purpose of the Study:

To develop a PEGylated liposome system for enhanced systemic delivery of PEI/oligodeoxynucleotide (ODN) polyplexes.
To evaluate the biodistribution and circulation time of ODN delivered via PEGylated liposomes.

Main Methods:

PEI/ODN polyplexes were encapsulated into PEGylated liposomes (PSL) with high loading efficiency, forming virus-like structures (~130 nm).
Stability of PSL in serum and the pharmacokinetic profile of entrapped ODN after intravenous administration were assessed.
Targeting PSL with transferrin receptor antibodies was used to redirect biodistribution.

Main Results:

PEGylated liposomes entrapping PEI/ODN polyplexes demonstrated stability in serum.
PSL significantly slowed the clearance rate of ODN from systemic circulation compared to naked PEI/ODN.
Targeted PSL showed significant ODN accumulation in the brain via transferrin receptor.

Conclusions:

Encapsulating PEI/ODN polyplexes within long-circulating PEGylated liposomes offers a promising strategy for in vivo ODN delivery.
This approach overcomes limitations of PEI, enabling improved systemic circulation and targeted organ accumulation.