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Related Experiment Video

Updated: Jun 27, 2026

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images
08:52

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

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A rapid topographic mapping and eye alignment method using optical imaging in Macaque visual cortex.

H D Lu1, G Chen, D Y Ts'o

  • 1Dept of Psychology, 301 Wilson Hall, Vanderbilt University, Nashville, TN 37212, USA.

Neuroimage
|November 18, 2008
PubMed
Summary
This summary is machine-generated.

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We developed a rapid spot imaging method to map visual fields and align eyes for optical imaging experiments. This technique ensures accurate stimulus placement and eye convergence without invasive electrophysiology.

Area of Science:

  • Neuroscience
  • Optical Imaging
  • Visual System Research

Background:

  • Accurate eye alignment and field of view mapping are crucial for optical imaging experiments, especially in visual cortex studies.
  • Conventional methods may be limited by space constraints or the need for repeated electrode insertion.

Purpose of the Study:

  • To develop a non-invasive, rapid, and repeatable spot imaging method for mapping the field of view and assessing eye convergence.
  • To facilitate precise stimulus placement for optical imaging procedures, such as studying ocular disparity responses.

Main Methods:

  • Utilized small (0.2-0.5 degrees) spots to map the field of view by imaging cortical responses at different positions.
  • Assessed eye convergence by presenting two spots, one to each eye, and measuring their relative positions with 0.1 degrees resolution.

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Investigating Object Representations in the Macaque Dorsal Visual Stream Using Single-unit Recordings
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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Related Experiment Videos

Last Updated: Jun 27, 2026

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images
08:52

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

Published on: May 23, 2019

Investigating Object Representations in the Macaque Dorsal Visual Stream Using Single-unit Recordings
07:08

Investigating Object Representations in the Macaque Dorsal Visual Stream Using Single-unit Recordings

Published on: August 1, 2018

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Main Results:

  • The spot imaging method allows for rapid mapping of the field of view.
  • Eye convergence can be accurately assessed, enabling precise alignment for subsequent optical imaging.

Conclusions:

  • This spot imaging technique provides a valuable tool for optimizing optical imaging experiments in neuroscience.
  • It enhances experimental efficiency and accuracy by ensuring proper eye alignment and stimulus positioning.