Related Experiment Video
Updated: Jun 27, 2026

Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model
Published on: December 14, 2016
Biofilm formation by persistent and non-persistent isolates of Staphylococcus epidermidis from a neonatal intensive
1Microbiology Department, Faculty of Biological Sciences, Shahid Beheshti University, Evin, Tehran, Iran. f-eftekhar@sbu.ac.ir
Abstract:
Correlation of biofilm formation and presence of the icaADBC genes was studied in 161 clinical isolates of S. epidermidis from persistent and non-persistent neonatal infections. Biofilm formation was compared in trypticase soy broth with or without added glucose (0.5-9.23%), and total parenteral nutrition (TPN) containing 9.23% glucose. Detection of icaADBC genes was carried out using polymerase chain reaction and ica-specific primers. Quantitative biofilm formation for all isolates was highest in the presence of 1% glucose, followed by trypticase soy broth and TPN. There was no significant difference between the amount of biofilm formed by persistent and non-persistent isolates under different test conditions. In contrast, 70% of the persistent isolates produced biofilm in TPN compared to the 56.3% of the non-persistent group. Neither the persistent bacterial phenotype nor presence of the icaADBC operon was correlated with biofilm formation.
More Related Videos
Related Concept Videos
Biofilms
Development of the Oral Microbiota
Staphylococcal Skin Infections

