Related Experiment Video
Updated: Jun 27, 2026

Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions
Published on: March 20, 2021
Retinoic acid receptor-related orphan receptor alpha regulates a subset of cone genes during mouse retinal
Hiroki Fujieda1, Rod Bremner, Alan J Mears
1Department of Anatomy, School of Medicine, Tokyo Women's Medical University, Tokyo, Japan. hfujieda@research.twmu.ac.jp
Abstract:
Color vision is supported by retinal cone photoreceptors that, in most mammals, express two photopigments sensitive to short (S-opsin) or middle (M-opsin) wavelengths. Expression of the Opn1sw and Opn1mw genes, encoding S-opsin and M-opsin, respectively, is under the control of nuclear receptors, including thyroid hormone receptor beta2 (TRbeta2), retinoid X receptor gamma (RXRgamma), and RORbeta, a member of the retinoic acid receptor-related orphan receptor (ROR) family. We now demonstrate that RORalpha, another member of the ROR family, regulates Opn1sw, Opn1mw, as well as Arr3 (cone arrestin) in the mouse retina. RORalpha expression is detected in cones by postnatal day 3 and maintained through adulthood. The retinas of staggerer mice, carrying a null mutation of RORalpha, show significant down-regulation of Opn1sw, Opn1mw, and Arr3. RORalpha acts in synergy with cone-rod homeobox transcription factor (Crx), to activate the Opn1sw promoter in vitro. Chromatin immunoprecipitation assays reveal that RORalpha directly binds to the Opn1sw promoter, Opn1mw locus control region, and the Arr3 promoter in vivo. Our data suggest that RORalpha plays a crucial role in cone development by directly regulating multiple cone genes.
More Related Videos
Related Concept Videos
The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The Retina
Photoreceptors and Visual Pathways
Anatomy of the Eyeball

