Standardized high-throughput evaluation of cell-based compound screens
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Published on: March 10, 2017
Peter Frommolt1, Roman K Thomas
1Institute of Medical Statistics, Informatics and Epidemiology, University of Köln, Köln, Germany. peter.frommolt@uni-koeln.de
Background:
High-throughput screening of pharmaceutical compound activity in tissue culture experiments requires time-consuming repeated analysis of the large amounts of data generated. Automation of the evaluation procedure and assessment of measurement accuracy can save time and improve the comparability of results.
Results:
We present a tool for simultaneous evaluation of an arbitrary number of compound screens including a standardized statistical validation. It is provided as a novel R package with a Tcl/Tk-based GUI for convenient use in the lab and runs on usual platforms like Linux, Windows and Mac OS. In a compound screen of lung cancer cells, the tool was successfully and efficiently applied for data analysis.
Conclusion:
The package provides an efficient and intuitive platform for automatic evaluation of compound screens, improving the performance and standardization of data analysis.
