Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Covalently Linked Protein Regulators

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Chromatin Position Affects Gene Expression

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

SIRT2 antagonizes MOF function during mitotic entry.

Science advances·2026

Same author

CRONDEX: a web-based platform for exploring links between chromatin-related genes and neurodevelopmental disorders.

Clinical epigenetics·2026

Same author

Epigenetic regulation of serine biosynthesis by PHF8 during neurogenesis.

EMBO reports·2026

Same author

Evaluation of enzyme activity predictions for variants of unknown significance in Arylsulfatase A.

Human genetics·2025

Same author

QAFI: a novel method for quantitative estimation of missense variant impact using protein-specific predictors and ensemble learning.

Human genetics·2024

Same author

PHF2-mediated H3K9me balance orchestrates heterochromatin stability and neural progenitor proliferation.

EMBO reports·2024

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Autoacetylation regulates P/CAF nuclear localization.

Noemí Blanco-García¹, Elena Asensio-Juan, Xavier de la Cruz

¹Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Baldiri i Reixac 15-21, Parc Cientific de Barcelona, E-08028 Barcelona, Spain.

The Journal of Biological Chemistry

|November 19, 2008

Summary

Protein acetylation, a key posttranslational modification, influences protein function. This study reveals how P/CAF autoacetylation controls its nuclear entry, impacting cellular processes like apoptosis.

More Related Videos

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Assays for Validating Histone Acetyltransferase Inhibitors

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

Related Experiment Videos

Last Updated: Jun 27, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Assays for Validating Histone Acetyltransferase Inhibitors

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Acetylation is a crucial posttranslational modification regulating protein function.
The acetyltransferase P/CAF undergoes autoacetylation and acetylation by p300.
P/CAF autoacetylation affects protein localization and activity.

Purpose of the Study:

To investigate the role of P/CAF autoacetylation in regulating its subcellular distribution.
To understand the impact of deacetylation on P/CAF localization.
To explore the connection between P/CAF localization and apoptosis.

Main Methods:

Analysis of P/CAF mutants lacking histone acetyltransferase activity.
Assessment of P/CAF subcellular distribution following autoacetylation and deacetylation.
Investigation of P/CAF localization during apoptosis.

Main Results:

A P/CAF mutant lacking histone acetyltransferase activity accumulated in the cytoplasm.
Non-autoacetylated P/CAF was enriched in the cytoplasmic fraction.
Deacetylation by HDAC3, HDAC1, HDAC2, or HDAC4 led to cytoplasmic P/CAF accumulation.
P/CAF was observed to accumulate in the cytoplasm during apoptosis.

Conclusions:

Autoacetylation of P/CAF is a critical mechanism controlling its nuclear translocation.
Deacetylation by specific HDACs promotes P/CAF cytoplasmic localization.
P/CAF cytoplasmic accumulation during apoptosis suggests a novel regulatory pathway.