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Three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina
M B Sherman1, E V Orlova, E A Smirnova
1Institute of Crystallography, USSR Academy of Sciences, Moscow.
Journal of Bacteriology
|April 1, 1991
Abstract:
The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped complex containing six each of the large (62-kDa) and small (26-kDa) subunits. The complex is very open, with six well-separated dumbbell-shaped masses surrounding a large cylindrical hole. Each dumbbell is interpreted as consisting of one large and one small subunit.