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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jun 27, 2026

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium
10:19

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

Published on: June 24, 2014

MicroRNA expression patterns and function in endodermal differentiation of human embryonic stem cells.

Galit Tzur1, Asaf Levy, Eti Meiri

  • 1Goldyne Savad Institute of Gene Therapy, Hadassah University Hospital, Jerusalem, Israel.

Plos One
|November 19, 2008
PubMed
Summary
This summary is machine-generated.

Human embryonic stem cells (hESC) show unique microRNA (miRNA) patterns during differentiation. While specific miRNAs correlate with endodermal gene expression, overexpressing miR-122 did not induce endodermal differentiation but delayed overall hESC development.

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08:37

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Published on: October 9, 2020

Area of Science:

  • Developmental Biology
  • Stem Cell Research
  • Molecular Genetics

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression.
  • Human embryonic stem cells (hESC) are valuable models for studying early human development.
  • Understanding miRNA roles in hESC differentiation is crucial for developmental studies.

Purpose of the Study:

  • To investigate unique miRNA expression patterns in differentiated hESC.
  • To determine the effect of endoderm-specific miRNA overexpression on hESC differentiation.

Main Methods:

  • Microarray and quantitative RT-PCR were used to profile miRNA expression in undifferentiated and differentiated hESC.
  • Lentiviral vectors were employed to overexpress liver-specific miR-122.
  • Genomewide gene microarrays analyzed the impact of miR-122 overexpression on hESC differentiation.

Main Results:

  • Three novel miRNAs were identified in both undifferentiated and differentiated hESC.
  • miR-24 and miR-10a were significantly upregulated upon differentiation, targeting genes that inhibit endodermal differentiation.
  • Overexpression of miR-122 did not induce endodermal differentiation but delayed general hESC differentiation.

Conclusions:

  • Specific miRNA expression correlates with gene expression during hESC differentiation.
  • miRNAs play a significant role in the endodermal differentiation of hESC.
  • Further research is needed to elucidate the precise mechanisms of miRNA-mediated differentiation.