Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Blood Typing

Blood Typing

Understanding an individual's blood group is a critical component of transfusion medicine. It ensures compatibility in blood transfusions, organ transplants, and even during pregnancy. Determining these blood groups involves the ABO and Rh blood typing systems, utilizing specific antigens and corresponding anti-sera to identify an individual's blood type.
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target antigens A,...

Blood Types

Blood Types

Human blood is classified into different types based on the presence of antigens on the red blood cell's surface and antibodies in the plasma. Proper identification of blood type is essential for successful blood transfusion. The International Society of Blood Transfusion has identified 38 human blood types based on the surface antigens on the red blood cells. The most common types are ABO, Rh, and MNS blood types.
ABO blood group
ABO antigens are glycoproteins encoded by genes present on...

The ABO Blood Group

The ABO Blood Group

The ABO blood group system is a critical element of transfusion medicine, essential for determining blood compatibility in transfusions and organ transplants. It is based on specific antigens, or agglutinogens, present on the surface of red blood cells (RBCs) and corresponding antibodies, or agglutinins, in the blood plasma.
Antigens in the ABO Blood Group System
Antigens are substances that can trigger an immune response, leading to the production of antibodies. In the ABO blood group system,...

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Blood Transfusion

Blood Transfusion

Blood transfusion is a critical medical procedure that saves lives and treats various medical conditions. It involves transferring blood from a donor to a recipient. This process requires a thorough understanding of the ABO blood group system and its associated antigens and antibodies.
Blood Transfusion Overview
A blood transfusion is a medical procedure used to replace blood lost due to injury, surgery, or to treat conditions such as anemia or cancer. During a transfusion, donor blood is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

RHCE genotyping using next generation sequencing: Allele specific reference sequences.

Transfusion·2024

Same author

Clinical Validation of a Prenatal Cell-Free DNA Screening Test for Fetal RHD in a Large U.S. Cohort.

Obstetrics and gynecology·2024

Same author

Investigation of Wright Blood Group Alleles and Genotypes in Malaria-Endemic Area in Southwestern Saudi Arabia.

International journal of general medicine·2024

Same author

Lysophosphatidylcholine Acetyltransferase 2 (<i>LPCAT2</i>) Influences the Gene Expression of the Lipopolysaccharide Receptor Complex in Infected RAW264.7 Macrophages, Depending on the <i>E. coli</i> Lipopolysaccharide Serotype.

Biology·2024

Same author

Rh Blood Group D Antigen Genotyping Using a Portable Nanopore-based Sequencing Device: Proof of Principle.

Clinical chemistry·2022

Same author

Next-generation sequencing of 35 RHD variants in 16 253 serologically D- pregnant women in the Finnish population.

Blood advances·2020

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Large-scale blood group genotyping: clinical implications.

¹Centre for Research in Biomedicine and UWE, Bristol Genomics Research Institute, Faculty of Health and Life Sciences, University of the West of England, Bristol, UK. neil.avent@uwe.ac.uk

British Journal of Haematology

|November 20, 2008

Summary

Blood group genotyping is advancing with high-throughput systems, offering comprehensive data beyond traditional serology. This technology shows promise for routine blood banking and prenatal diagnostics.

More Related Videos

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

Related Experiment Videos

Last Updated: Jun 27, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

Area of Science:

Transfusion Medicine
Genetics
Immunology

Background:

Molecular basis of blood group antigen expression is well-understood.
Genotyping currently has a limited role in routine blood banking but is crucial for prenatal diagnostics.
High-throughput genotyping systems are emerging for large-scale DNA analysis.

Purpose of the Study:

To review the current state of blood group genotyping.
To predict the future evolution of genotyping in blood banking.
To highlight the potential of DNA-based typing to replace serology.

Main Methods:

Review of current literature and emerging high-throughput genotyping technologies.
Analysis of the impact of genotyping in prenatal diagnosis and potential for blood banks.
Comparison of DNA-based typing standardization with traditional serological methods.

Main Results:

Genotyping provides more comprehensive blood group information than serology alone.
DNA-based typing is easier to standardize and has the potential to replace serology.
High-throughput systems enable mass-scale genotyping from donor and patient DNA.

Conclusions:

Blood group genotyping is poised to become a frontline diagnostic tool in blood banks.
Advancements in high-throughput systems will enhance its utility in transfusion medicine.
Genotyping offers a more robust and standardized approach to blood group determination.