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Updated: Jun 27, 2026

High-Throughput Metabolic Profiling for Model Refinements of Microalgae
Published on: December 4, 2021
A new divergent type of eukaryotic methionine adenosyltransferase is present in multiple distantly related secondary
Gabino F Sanchez-Perez1, Vladimir Hampl, Alastair G B Simpson
1Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Canada B3H 1X5. gabino.sanchez.perez@gmail.com
Abstract:
S-adenosylmethionine is one of the most important metabolites in living cells and is synthesized in a single reaction catalyzed by methionine adenosyltransferase (MAT). At the sequence and structural level, this enzyme is one of the most conserved proteins known. Here we show that some representatives of three distantly related eukaryotic lineages--dinoflagellates, haptophytes, and euglenids--possess a highly divergent type of MAT, which we call MATX. Even though MATX contains all the sites known to be involved in catalysis and the association of monomers, it also has four insertions throughout the protein that are not observed in other MAT homologs. The phylogenetic distribution and affinities of MATX suggest that it originated in a single eukaryotic lineage and was spread via multiple events of eukaryote-to-eukaryote lateral gene transfer. We suggest a tentative model in which the origin of MATX is connected with the progression of secondary endosymbiosis.
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