Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Are we safe? Exploring psychological factors affecting radiation risk perception of dwellers in old uranium mining quarters.

Annals of the ICRP·2026
Same author

Relationship between hair cortisol and plasma heat shock proteins in response to heat stress in tolerant and sensitive cattle breeds.

Scientific reports·2026
Same author

Addressing Critical Fungal Pathogens Under a One Health Perspective: Key Insights from the Portuguese Association of Medical Mycology.

Mycopathologia·2025
Same author

High dietary total energy intake and energy density are associated with girls' breast density during puberty.

European journal of nutrition·2025
Same author

Serum and saliva immunoglobulin (immunoglobulin G, immunoglobulin A, and immunoglobulin M) dynamics in newborn calves and their association with health status during the first week of life: An exploratory study.

Journal of dairy science·2025
Same author

Central nervous system vascular complications associated with the acute form of steroid-responsive meningitis-arteritis.

Veterinary journal (London, England : 1997)·2025
Same journal

BioImageIT: A novel python-based architecture for reproducible bio-image workflows.

Journal of microscopy·2026
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
See all related articles

Related Experiment Video

Updated: Jun 27, 2026

Laser Cell Ablation in Intact Drosophila Larvae Reveals Synaptic Competition
05:27

Laser Cell Ablation in Intact Drosophila Larvae Reveals Synaptic Competition

Published on: July 26, 2024

Decrease in laser ablation threshold for epithelial tissue microsurgery in a living Drosophila embryo during dorsal

A K N Thayil1, A Pereira, M Mathew

  • 1ICFO - Institut de Ciències Fotòniques, Mediterranean Technology Park, 08860 Castelldefels, Barcelona, Spain.

Journal of Microscopy
|November 20, 2008
PubMed
Summary
This summary is machine-generated.

This study demonstrates laser microsurgery on Drosophila embryos using two-photon microscopy. Ablation of GFP-labeled tissue revealed insights into developmental forces and cellular repair mechanisms during embryogenesis.

More Related Videos

Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
12:35

Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula
09:50

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

Published on: July 15, 2021

Related Experiment Videos

Last Updated: Jun 27, 2026

Laser Cell Ablation in Intact Drosophila Larvae Reveals Synaptic Competition
05:27

Laser Cell Ablation in Intact Drosophila Larvae Reveals Synaptic Competition

Published on: July 26, 2024

Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
12:35

Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula
09:50

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

Published on: July 15, 2021

Area of Science:

  • Developmental Biology
  • Biophysics
  • Microscopy and Imaging

Background:

  • Precise laser microsurgery is crucial for studying cellular and tissue dynamics during embryonic development.
  • Understanding tissue mechanics and cellular responses to injury is fundamental in developmental biology.

Purpose of the Study:

  • To perform combined imaging and laser tissue ablation on living Drosophila Melanogaster embryos.
  • To investigate the role of Green Fluorescent Protein (GFP) in laser ablation efficiency.
  • To analyze tissue mechanics during embryonic dorsal closure and subsequent healing.

Main Methods:

  • Utilized a two-photon fluorescence microscope with tightly focused near-infrared femtosecond laser pulses.
  • Performed laser ablation on both labeled (GFP) and unlabeled Drosophila embryos during and after dorsal closure.
  • Quantified energy deposition required for tissue ablation.

Main Results:

  • Lower energy deposition was required for ablating GFP-labeled tissue, indicating multiphoton absorption by GFP.
  • Reduced energy deposition was observed during dorsal closure, suggesting increased tissue tensile forces.
  • Increased actin activity was detected near laser-induced wounds during tissue healing.

Conclusions:

  • Laser ablation efficiency in Drosophila embryos is influenced by the presence of fluorescent proteins like GFP.
  • Dorsal closure involves significant tensile forces within the embryonic tissue.
  • Embryonic tissues exhibit active cellular responses, including actin remodeling, to repair laser-induced damage.