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Updated: Jun 27, 2026

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
Encystment of Azotobacter nigricans grown diazotrophically on kerosene as sole carbon source
Gabriela García-Esquivel1, Graciano Calva-Calva, Ronald Ferrera-Cerrato
1Biotechnology and Bioengineering Department, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, C. P. 07360 Mexico D. F., Mexico.
Abstract:
Encystment of Azotobacter nigricans was induced by its diazotrophic cultivation on kerosene. Its growth and nitrogenase activity were affected by kerosene in comparison to cultures grown on sucrose. Electron microscopy of vegetative cells showed that when nitrogenase activity was higher and the poly-beta-hydroxybutyrate granules were not present to a significant extent, peripheral bodies were abundant. After 8 days of culture on kerosene, the presence of cysts with intracellular bunches of poly-beta-hydroxybutyrate granules was observed. Germination of cysts bears germinating multicelled yet unbroken capsule cysts with up to three cells inside. This is the first report of encystment induction of Azotobacter species grown on kerosene.
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